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rnp buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher rnp buffer
    Rnp Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnp+buffer/HEPES/pm29668265__ja8b01551_si_001-506-9-38
    Average 99 stars, based on 1 article reviews
    rnp buffer - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    CRISPR:

    Article Title: PEX1 G843D remains functional in peroxisome biogenesis but is rapidly degraded by the proteasome
    Article Snippet: .. For CRISPR-Cas9 editing, cells were nucleofected by combining RNP buffer (100 mM HEPES, 750 mM KCl, 25 mM MgCl 2 , 25% glycerol, and 5 mM TCEP), 2.5 μM sgRNA, 2 μM Cas9 (purchased from Berkeley MacroLab), and 5 μM dsDNA donor, and then adding this mixture to 200k cells washed with DPBS (Gibco #14190-144) and resuspended in nucleofection buffer SE (Lonza #V4XC-1024). .. Reaction mixtures were then electroporated in 4D Nucleocuvettes (Lonza) and subsequently recovered with pre-warmed media in culture dishes.

    Article Title: PEX1 G843D remains functional in peroxisome biogenesis but is rapidly degraded by the proteasome.
    Article Snippet: .. For CRISPR-Cas9 editing, cells were nucleofected by combining RNP buffer (100 mM HEPES, 750 mM KCl, 25 mM MgCl2, 25% glycerol, and 5 mM TCEP), 2.5 μM sgRNA, 2 μM Cas9 (purchased from Berkeley MacroLab), and 5 μM dsDNA donor, and then adding this mixture to 200k cells washed with DPBS (Gibco #14190-144) and resuspended in nucleofection buffer SE (Lonza #V4XC-1024). .. Reaction mixtures were then electroporated in 4D Nucleocuvettes (Lonza) and subsequently recovered with pre-warmed media in culture dishes.

    Gentle:

    Article Title: S1 Receptor-Mediated Delivery of CRISPR-Cas9 Endonuclease for Cell Type Specific Gene Editing
    Article Snippet: .. The coincubation mixtures were made by adding 20 μL RNP buffer [20 mM HEPES-NaOH pH 7.45, 150 mM NaCl, 10% (v/v) glycerol and 1 mM MgCl2] and 20 μL 12.5 μM RNP (250 pmol) to 500 μL OptiMEM (Thermofisher 31985062), followed by mixing by gentle pipetting and 5 min. incubation at ambient temperatures. ..

    Article Title: S1 Receptor-Mediated Delivery of CRISPR-Cas9 Endonuclease for Cell Type Specific Gene Editing
    Article Snippet: .. The coincubation mixtures were made by adding 20 μL RNP buffer [20 mM HEPES-NaOH pH 7.45, 150 mM NaCl, 10% (v/v) glycerol and 1 mM MgCl2] and 20 μL 12.5 μM RNP (250 pmol) to 500 μL OptiMEM (Thermofisher 31985062), followed by mixing by gentle pipetting and 5 min incubation at ambient temperatures. ..

    Incubation:

    Article Title: S1 Receptor-Mediated Delivery of CRISPR-Cas9 Endonuclease for Cell Type Specific Gene Editing
    Article Snippet: .. The coincubation mixtures were made by adding 20 μL RNP buffer [20 mM HEPES-NaOH pH 7.45, 150 mM NaCl, 10% (v/v) glycerol and 1 mM MgCl2] and 20 μL 12.5 μM RNP (250 pmol) to 500 μL OptiMEM (Thermofisher 31985062), followed by mixing by gentle pipetting and 5 min. incubation at ambient temperatures. ..

    Article Title: Trm112 Is Required for Bud23-Mediated Methylation of the 18S rRNA at Position G1575
    Article Snippet: The resuspension buffer consisted of polyvinylpyrrolidone 40 (PVP-40) at 1.2%, HEPES (pH 7.4) at 20 mM, Sigma protease inhibitor cocktail at 1:100, solution P at 1:100 (pepstatin A at 2 mg, phenylmethylsulfonyl fluoride [PMSF] at 90 mg, and ethanol at 5 ml), and DTT (1 M) at 1:1,000. .. The grindate was resuspended in RNP buffer (HEPES [pH 7.4] at 20 mM, potassium acetate [KOAc] at 110 mM, Triton X-100 at 0.5%, Tween 20 at 0.1%, Ambion SuperRNAsin at 1:5,000, Sigma antifoam at 1:5,000, solution P at 1:100, and NaCl at 150 mM) and incubated with rabbit IgG-conjugated magnetic beads (Dynal) for 1 h at 4°C. ..

    Article Title: Group II intron as cold sensor for self-preservation and bacterial conjugation
    Article Snippet: The quantitation was done using ImageQuant (GE Healthcare). .. The intron RNA and the IEP were mixed as described above and incubated in the RNP buffer (100 mM KCl, 5 mM MgCl 2 , 20 mM Tris–HCl (pH 7.5) and 1 U/μl murine RNase-inhibitor) at 10°C or 30°C for 3 h. The mixture was then analyzed on a 4% native polyacrylamide gel run at 4°C followed by SYPRO Ruby staining (Thermo Fisher) according to manufacturer's instructions. ..

    Article Title: The catalytic activity of the translation termination factor methyltransferase Mtq2-Trm112 complex is required for large ribosomal subunit biogenesis
    Article Snippet: The resuspension buffer consisted of: 1.2% PVP-40, 20 mM HEPES pH 7.4, 1:100 Sigma protease inhibitor cocktail, and 1:100 solution P (2 mg pepstatin A and 90 mg PMSF in 5 ml ethanol) and 1 mM DTT. .. The extract was resuspended in RNP buffer (20 mM HEPES pH 7.4, 110 mM KOAc, 0.5% Triton X-100, 0.1% Tween-20, 1:5000 Ambion SuperRNAsin, 1:5000 Sigma antifoam, 1:100 solution P, 150 mM NaCl) and incubated with prepared magnetic beads with rabbit IgG (Dynal) for 1 h at 4°C. ..

    Article Title: S1 Receptor-Mediated Delivery of CRISPR-Cas9 Endonuclease for Cell Type Specific Gene Editing
    Article Snippet: .. The coincubation mixtures were made by adding 20 μL RNP buffer [20 mM HEPES-NaOH pH 7.45, 150 mM NaCl, 10% (v/v) glycerol and 1 mM MgCl2] and 20 μL 12.5 μM RNP (250 pmol) to 500 μL OptiMEM (Thermofisher 31985062), followed by mixing by gentle pipetting and 5 min incubation at ambient temperatures. ..

    Article Title: Trm112 Is Required for Bud23-Mediated Methylation of the 18S rRNA at Position G1575
    Article Snippet: The resuspension buffer consisted of polyvinylpyrrolidone 40 (PVP-40) at 1.2%, HEPES (pH 7.4) at 20 mM, Sigma protease inhibitor cocktail at 1:100, solution P at 1:100 (pepstatin A at 2 mg, phenylmethylsulfonyl fluoride [PMSF] at 90 mg, and ethanol at 5 ml), and DTT (1 M) at 1:1,000. .. The grindate was resuspended in RNP buffer (HEPES [pH 7.4] at 20 mM, potassium acetate [KOAc] at 110 mM, Triton X-100 at 0.5%, Tween 20 at 0.1%, Ambion SuperRNAsin at 1:5,000, Sigma antifoam at 1:5,000, solution P at 1:100, and NaCl at 150 mM) and incubated with rabbit IgG-conjugated magnetic beads (Dynal) for 1 h at 4°C. ..

    Magnetic Beads:

    Article Title: Trm112 Is Required for Bud23-Mediated Methylation of the 18S rRNA at Position G1575
    Article Snippet: The resuspension buffer consisted of polyvinylpyrrolidone 40 (PVP-40) at 1.2%, HEPES (pH 7.4) at 20 mM, Sigma protease inhibitor cocktail at 1:100, solution P at 1:100 (pepstatin A at 2 mg, phenylmethylsulfonyl fluoride [PMSF] at 90 mg, and ethanol at 5 ml), and DTT (1 M) at 1:1,000. .. The grindate was resuspended in RNP buffer (HEPES [pH 7.4] at 20 mM, potassium acetate [KOAc] at 110 mM, Triton X-100 at 0.5%, Tween 20 at 0.1%, Ambion SuperRNAsin at 1:5,000, Sigma antifoam at 1:5,000, solution P at 1:100, and NaCl at 150 mM) and incubated with rabbit IgG-conjugated magnetic beads (Dynal) for 1 h at 4°C. ..

    Article Title: The catalytic activity of the translation termination factor methyltransferase Mtq2-Trm112 complex is required for large ribosomal subunit biogenesis
    Article Snippet: The resuspension buffer consisted of: 1.2% PVP-40, 20 mM HEPES pH 7.4, 1:100 Sigma protease inhibitor cocktail, and 1:100 solution P (2 mg pepstatin A and 90 mg PMSF in 5 ml ethanol) and 1 mM DTT. .. The extract was resuspended in RNP buffer (20 mM HEPES pH 7.4, 110 mM KOAc, 0.5% Triton X-100, 0.1% Tween-20, 1:5000 Ambion SuperRNAsin, 1:5000 Sigma antifoam, 1:100 solution P, 150 mM NaCl) and incubated with prepared magnetic beads with rabbit IgG (Dynal) for 1 h at 4°C. ..

    Article Title: Trm112 Is Required for Bud23-Mediated Methylation of the 18S rRNA at Position G1575
    Article Snippet: The resuspension buffer consisted of polyvinylpyrrolidone 40 (PVP-40) at 1.2%, HEPES (pH 7.4) at 20 mM, Sigma protease inhibitor cocktail at 1:100, solution P at 1:100 (pepstatin A at 2 mg, phenylmethylsulfonyl fluoride [PMSF] at 90 mg, and ethanol at 5 ml), and DTT (1 M) at 1:1,000. .. The grindate was resuspended in RNP buffer (HEPES [pH 7.4] at 20 mM, potassium acetate [KOAc] at 110 mM, Triton X-100 at 0.5%, Tween 20 at 0.1%, Ambion SuperRNAsin at 1:5,000, Sigma antifoam at 1:5,000, solution P at 1:100, and NaCl at 150 mM) and incubated with rabbit IgG-conjugated magnetic beads (Dynal) for 1 h at 4°C. ..

    Staining:

    Article Title: Group II intron as cold sensor for self-preservation and bacterial conjugation
    Article Snippet: The quantitation was done using ImageQuant (GE Healthcare). .. The intron RNA and the IEP were mixed as described above and incubated in the RNP buffer (100 mM KCl, 5 mM MgCl 2 , 20 mM Tris–HCl (pH 7.5) and 1 U/μl murine RNase-inhibitor) at 10°C or 30°C for 3 h. The mixture was then analyzed on a 4% native polyacrylamide gel run at 4°C followed by SYPRO Ruby staining (Thermo Fisher) according to manufacturer's instructions. ..



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